dnaman program Search Results


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Program Dnaman 6.030, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Alignment Program Dnaman, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Computer Program Dnaman, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sequence Assembly Function Of The Dnaman Program, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnaman program  (Gallus BioPharmaceuticals)
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The RNA demethylase activity <t>of</t> <t>FTO</t> is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Dnaman Program, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein analysis program dnaman  (Lynnon corporation)
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The RNA demethylase activity <t>of</t> <t>FTO</t> is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Protein Analysis Program Dnaman, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnaman 7 program  (Lynnon corporation)
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The RNA demethylase activity <t>of</t> <t>FTO</t> is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Dnaman 7 Program, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multisequence alignment program dnaman 5.2.9  (Lynnon corporation)
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Lynnon corporation multisequence alignment program dnaman 5.2.9
The RNA demethylase activity <t>of</t> <t>FTO</t> is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Multisequence Alignment Program Dnaman 5.2.9, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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program dnaman versión 4.03  (Lynnon corporation)
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Lynnon corporation program dnaman versión 4.03
The RNA demethylase activity <t>of</t> <t>FTO</t> is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Program Dnaman Versión 4.03, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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web-based pcr primer designing program dnaman  (Lynnon corporation)
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The RNA demethylase activity <t>of</t> <t>FTO</t> is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Web Based Pcr Primer Designing Program Dnaman, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer program dnaman version 5.2.10  (Lynnon corporation)
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The RNA demethylase activity <t>of</t> <t>FTO</t> is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Computer Program Dnaman Version 5.2.10, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer program dnaman 4.1  (Lynnon corporation)
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The RNA demethylase activity <t>of</t> <t>FTO</t> is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls. " width="250" height="auto" />
Computer Program Dnaman 4.1, supplied by Lynnon corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The RNA demethylase activity of FTO is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site

Journal: iScience

Article Title: FTO promotes innate immunity by controlling NOD1 expression via m 6 A-YTHDF2 manner in teleost

doi: 10.1016/j.isci.2022.105646

Figure Lengend Snippet: The RNA demethylase activity of FTO is required for promoting innate immunity (A) Sequence alignment on both sides of the mutation site "R" in FTO in H. sapiens (no. NP_001073901.1) , M. musculus (no. NP_036066.2) , G. gallus (no. NP_001172076.1) , X. laevis (no. NP_001087481.1) , D. rerio (no. XP_001345910.4) , M. miiuy (no. OP168916) and C. milii (no. XP_007887772.2). (B) FTO significantly decreased the mRNA m 6 A content. MKC cells were transfected with si-Ctrl or si-FTO and vector or FTO or FTO-mut plasmid for 48 h, then the m 6 A level was measured by colorimetry. (C) mRNA expression of FTO, IFN-1, and TNF-α in MKC cells transfected with FTO or FTO-m. (D) FTO and FTO-mut overexpressed MKC cells seeded in 48-well plates overnight were treated with SCRV at the dose indicated for 48 h. Then, cell monolayers were fixed with 4% paraformaldehyde and stained with 1% crystal violet. (E) MKC cells were transfected with pcDNA3.1 vector and FTO or FTO-mut expression plasmid for 24 h, then infected with SCRV for 24 h. The qPCR analysis was conducted for intracellular and supernatant SCRV RNA levels. (F) MKCs were transfected with pcDNA3.1 vector and FTO or FTO-mut plasmid, then infected with FITC-labeled V. anguillarum , and then examined by using a fluorescence microscope. Scale bars, 20 mm; original magnification ×400. All data presented as the means ± SE from at least three independent triplicated experiments. ∗∗, p < 0.01; ∗, p < 0.05 versus the controls.

Article Snippet: In order to find out the site that can affect the activity of FTO demethylase in the miiuy croaker, DNAMAN program was used to perform multiple sequence alignment from Homo sapiens , Mus musculus , Gallus gallus , Xenopus laevis , Danio rerio , Miichthys miiuy and Callorhinchus milii .

Techniques: Activity Assay, Sequencing, Mutagenesis, Transfection, Plasmid Preparation, Colorimetric Assay, Expressing, Staining, Infection, Labeling, Fluorescence, Microscopy